December 1, 2022

Blog @ Munaf Sheikh

Latest news from tech-feeds around the world.

Futurism: Vacuum method identifies animals with DNA in the air


Using a new technique, researchers are able to vacuum animal DNA from the air and identify the species that live nearby.

Identifying the animal species that live in a particular area can be a lot of work. Usually it means spotting the animals with the naked eye or recording them using a camera trap. Their presence can also be identified via the traces they leave on the ground or in the form of, for example, feces. Common to all these methods, however, is that they are time-consuming.

Researchers tested the new method in the Copenhagen Zoo, where they have filtered the air in the okapi stables, the rainforest hall, and outside around the enclosures.

“We were amazed by the results. By vacuuming DNA from the air, we were able to identify 49 animal species, including mammals, birds, frogs, reptiles, and fish. In the rainforest hall, we even found traces of the sloth, the boa, and the guppies that live in a small pond,” says Kristine Bohmann, associate professor from the GLOBE Institute at the University of Copenhagen, who heads the study.

Associate Professor Kristine Bohmann collecting air samples near a sloth. (Credit: Christian Bendix)

“Outside we identified a lot of the animals that have access to outdoor spaces in this section of the park, including ostrich, guinea fowl, giraffe, zebra, impala, okapi, and rhinoceros.”

‘Air touches everything’

Researchers tested different methods for filtering animal DNA from the air: a so-called water vacuum, where DNA from the air is sucked into water, and a fan like the ones used to cool computers. The latter sucked the air into a filter, which then identified the DNA. The researchers tested two versions of the fan, the smallest of which was no larger than a golf ball. Using these three “vacuums,” the researchers collected a total of 40 air samples from the Copenhagen Zoo.

Most, but not all of the animal species identified by the researchers via airborne DNA live in captivity in the zoo. The researchers also identified DNA from species that live outside the zoo, including dogs, cats, water voles, yellow-necked mice, rats, and red squirrels. They even registered three species of fish fed to the animals in the zoo.

“It is difficult to work with air, because air touches everything. This means that the risk of getting polluted samples was significant. And we wanted to make sure the species we identified actually came from the zoo and not from our own laboratory. Therefore, we used a set of extremely strict environmental DNA methods. We even took samples from the air in the lab to make sure that it did not contain any animal DNA,” says postdoc Christina Lynggaard from the GLOBE Institute.

DNA vacuum may boost conservation

Concurrently with the Danish research team, a group of British researchers at Queen Mary University of London conducted a similar study in the Hamerton Zoo Park. They used a third method to collect more than 70 air samples from different parts of the park.

Here they found DNA from 25 different animal species, 17 of which lived in the park, while the rest were wild animals from the surrounding area, including hedgehogs, which in the UK are an endangered species. The two studies’ independent demonstration of airborne DNA for animal monitoring supports the potential of the method.

Animals leave DNA traces in their environment, and especially environmental DNA in water is a popular method for mapping species in aquatic environments. The two research teams now hope that airborne DNA will develop into its counterpart on dry land and, supported by more research, a strong tool for mapping the animals present in a given area.

“The loss of biodiversity globally means that we need new methods for monitoring animal species. Airborne environmental DNA has the potential to revolutionize our ability to map biodiversity on dry land and to monitor the composition of animal populations,” says Elizabeth Clare, assistant professor at York University, formerly at Queen Mary University of London.

“It also enables us to keep an eye on invasive species and to record the presence of species in areas that are difficult to access, e.g., caves and caverns.”

“I have to admit that I did not think we would succeed—and definitely not to this extent. Even though more research is required before we can utilize the full potential of airborne DNA, we believe it can change our ability to map the presence of animals completely, and that it will give us valuable knowledge in connection with e.g. the protection and preservation of biodiversity,” Bohmann says.

Both studies (study 1, study 2) appear in Current Biology.

The VILLUM Foundation’s VILLUM Experiment program funded the work.

Source: University of Copenhagen



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